ombjj
ombjj
11-07-2019
Biology
contestada
List the steps in isolating embryonic stem cells.
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Zucchinini
Zucchinini
11-07-2019
1. Cleavage stage embryo
2. Cultures blastocyst
3. Isolated inner cell mass
4. Irradiated mouse fibroblast feeder cells
5. Cells dissociated and replaced
6. New feeder cells
7. Established embryonic stem cells cultures
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