You transform an E. coli bacterium with the pGLO plasmid. After growing the +pGLO bacteria on an LB+Ampicillin+Arabinose plate, you see distinct colonies form after 16 hours. You check these colonies under the UV light and see that they are glowing a bright fluorescent green color. If you use a sterile loop and inoculate a new plate with LB+Ampicillin (coated on them, as usual) with these transformed E. coli will you see any growth? If there is growth, will it glow?
Expression of beta lactamase (gene that confers resistance to ampicillin) is constitutive in pGLO, but expression of GFP is dependent on the presence of arabinose
