sailormaries6110 sailormaries6110
  • 11-02-2020
  • Biology
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A binding protein binds to a ligand, L, with a Kd of 400 nM. How much ligand (in nM) is present when the fraction of ligand bound is 60%?

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olumidechemeng olumidechemeng
  • 13-02-2020

Answer:

600nM

Explanation:

  • From Concentration of Ligand = Ykd/1 - Y
  • where Kd = 400nM.
  • fraction of ligand bound Y = 0.60

  • Plugging the values of Y and Kd;
  • Concentration = 0.6 x 400 / 1 - 0.6
  • Concentration = 600 x 10^-9
  • = 600nM
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